A Study of Testosterone Metabolism using GC/C/IRMS
Objectives
The objective of this study is to determine the influence of testosterone administration on the 13C/12C isotopic ratios and to measure their evolution over time.
Introduction
Endogenous substances produced by the organism display 13C/12C isotopic ratios that reflect one’s dietary habits. They are thus specific for a given individual. Administration of anabolic steroids modifies these ratios, a finding that can be used to detect doping.
Methodology
- GC/C/IRMS analysis of urinary secretions over a period of 24 hours in 8 Caucasian individually that have ingested 80 mg of testosterone undecanoate;
- Determination of absolute isotopic ratios for several steroids that are detectable in the urine;
- Comparison between testosterone metabolites (affected by testosterone ingestion) and endogenous substances (not affected by testosterone ingestion);
- Determination of a detection window of doping in the test subjects.
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